PhD (HK)

Associate Professor of Department of Chemistry


Contact Information

Office: YEUNG-G6619
Phone: +852 3442-6347
Fax: +852 3442-0522
Email: yunwlam@cityu.edu.hk
Web: Personal Homepage Blog
ORCID ID: 0000-0001-8929-5965
Author ID:

Dr. Yun Wah Lam received his PhD training in the lab of Dr. Davina Opstelten at the University of Hong Kong. After receiving his PhD in 1997, he joined the group of Prof. Angus Lamond in Dundee, Scotland, where he developed an interest in the relationship of the architecture of mammalian cell nucleus and the regulation of gene expression. Lam uses live-cell imaging techniques and classical biochemical approaches to study protein localization and interactions in the cell nucleus. In parallel, he is involved in an international effort to map the human nucleolus proteome. Recently, in collaboration with Jens Andersen (Odense) and Matthias Mann (Munich), he adopted the technique of SILAC (Stable Isotope Labeling with Amino acids in Cell culture) to quantify, by mass spectrometry, the global dynamics of the human nucleolar proteome in response to changes in metabolic conditions. The resulting paper, having received over 100 citations in 24 months, was featured as the "ScienceWatch hot paper" in The Scientist magazine (March 2007). Lam was the recipient of the second prize of the Roche "Imagining the future" contest in 2006. He joined City University of Hong Kong in 2007.

Research Interests and Projects

Proteomics allow the large-scale identification of proteins in complex biological systems, but it often only produces static snapshots of proteomes that fail to describe and explain the dynamic nature of cellular behaviours. In contrast, modern cell imaging techniques allow the quantitative analysis of the spatial and temporal distribution of biomolecules in living cells, but only a relatively small number of proteins can be studied in one experiment.  Our lab strives to bring together these two powerful methodologies to tackle fundamental biological questions. To this end, we perform quantitative mass spectrometry to measure the flux of a large number of proteins through subcellular spaces and to track the proteomic changes within cellular complexes through time. In parallel, we record these dynamic cellular behaviours in live cells using time-lapse fluorescence microscopy. From these two angles, we aim at building coherent pictures of intracellular events under different growth and metabolic conditions. This lab aims to apply this dual approach to a range of topics, from basic cell biology to environmental sciences.

Project lists as principal investigator

Funded by UGC Sources

  • Subcellular distribution of microRNAs: Another dimension of regulation?
  • Proteomic study on nuclear reprogramming during cell differentiation
  • The Proteomic Landscape of the Human Nucleolus during Senescence

Funded by Non-UGC Sources

  • Teaching development grant:"Using programmed learning to help applied biology students to prepare for laboratory classes"(Completed)
  • New staff startup grant: “A novel approach for studying mammalian nuclear import using SILAC-based proteomics"(Completed)

Selected Publications

Full pulbications can be viewed here

  • Dong, Miao; Liang, Yimin; Ramalingam, Rajkumar; Tang, Sze Wing; Shen, Wei; Ye, Rui; Gopalakrishnan, Singaram; Au, Doris Wai Ting; Lam, Yun Wah. Proteomic characterization of the interactions between fish serum proteins and waterborne bacteria reveals the suppression of anti-oxidative defense as a serum-mediated antimicrobial mechanism. Fish & Shellfish Immunology. Elsevier. 2017.
  • Zhou, Shu Fan; Gopalakrishnan, Singaram; Xu, Yuan Hao; Yang, Jie; Lam, Yun Wah; Pang, Stella W. A Unidirectional Cell Switching Gate by Engineering Grating Length and Bending Angle. PloS one. Public Library of Science. 2016;11(1):e0147801.
  • Tang, QY; Qian, WX; Xu, YH; Gopalakrishnan, S; Wang, JQ; Lam, YW; Pang, SW. Control of cell migration direction by inducing cell shape asymmetry with patterned topography. Journal of Biomedical Materials Research Part A. Wiley Online Library. 2015;103(7):2383-2393.
  • Poon, Ellen; Keung, Wendy; Liang, YM; Ramalingam, Rajkumar; Yan, Bin; Zhang, Shaohong; Chopra, Anant; Moore, Jennifer; Herren, Anthony; Lieu, Deborah K. Proteomic analysis of human pluripotent stem cell-derived, fetal and adult ventricular cardiomyocytes reveals pathways crucial for cardiac metabolism and maturation. Circulation: Cardiovascular Genetics. Am Heart Assoc. 2015;CIRCGENETICS. 114.000918.
  • Tang, Qing Yuan; Tong, Wing Yin; Shi, Jue; Shi, Peng; Lam, Yun Wah; Pang, Stella W. Influence of engineered surface on cell directionality and motility. Biofabrication. IOP Publishing. 2014;6(1):15011.
  • Liang, Yi Min; Wang, Xian; Ramalingam, Rajkumar; So, Kin Yan; Lam, Yun Wah; Li, Zhou Fang. Novel nucleolar isolation method reveals rapid response of human nucleolar proteomes to serum stimulation. Journal of proteomics. Elsevier. 2012;77:521-530.
  • Li, Zhou Fang; Liang, Yi Min; Lau, Pui Ngan; Shen, Wei; Wang, Dai Kui; Cheung, Wing Tai; Xue, Chun Jason; Poon, Lit Man; Lam, Yun Wah. Dynamic localisation of mature microRNAs in Human nucleoli is influenced by exogenous genetic materials. PLoS One. Public Library of Science. 2013;8(8):e70869.
  • Babaei, Fatemeh; Ramalingam, Rajkumar; Tavendale, Amy; Liang, Yimin; Yan, Leo So Kin; Ajuh, Paul; Cheng, Shuk Han; Lam, Yun Wah. Novel blood collection method allows plasma proteome analysis from single zebrafish. Journal of proteome research. ACS Publications. 2013;12(4):1580-1590.
  • Cai, Xiaoning; Ramalingam, Rajkumar; San Wong, Hau; Cheng, Jinping; Ajuh, Paul; Cheng, Shuk Han; Lam, Yun Wah. Characterization of carbon nanotube protein corona by using quantitative proteomics. Nanomedicine: Nanotechnology, Biology and Medicine. Elsevier. 2013;9(5):583-593.
  • Tong, Wing Yin; Shen, Wei; Yeung, Connie WF; Zhao, Ying; Cheng, Shuk Han; Chu, Paul K; Chan, Danny; Chan, Godfrey CF; Cheung, Kenneth MC; Yeung, Kelvin WK. Functional replication of the tendon tissue microenvironment by a bioimprinted substrate and the support of tenocytic differentiation of mesenchymal stem cells. Biomaterials. Elsevier. 2012;33(31):7686-7698.
  • Dong, Hongjuan; Shen, Wei; Cheung, Myra Ting Wai; Liang, Yimin; Cheung, Hon Yeung; Allmaier, Günter; Au, Oscar Kin-Chung; Lam, Yun Wah. Rapid detection of apoptosis in mammalian cells by using intact cell MALDI mass spectrometry. Analyst. Royal Society of Chemistry. 2011;136(24):5181-5189.
  • Tong, WY; Liang, YM; Tam, Vivian; Yip, HK; Kao, YT; Cheung, KMC; Yeung, KWK; Lam, YW. Biochemical characterization of the cell-biomaterial interface by quantitative proteomics. Molecular & Cellular Proteomics. ASBMB. 2010;9(10):2089-2098.
  • Lam, Yun W; Evans, Vanessa C; Heesom, Kate J; Lamond, Angus I; Matthews, David A. Proteomics analysis of the nucleolus in adenovirus-infected cells. Molecular & Cellular Proteomics. ASBMB. 2010;9(1):117-130.