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Mutagenesis-based Evolutionary Engineering for Microbial Biobreeding Aiming at Application in Open Systems
Speaker Name
Prof. Xin-Hui XING
Speaker Detail

Professor and Deputy Director
MOE Key Laboratory of Industrial Biocatalysis
Department of Chemical Engineering
Center for Synthetic and Systems Biology
Tsinghua University, Beijing

Room 2100, 2/F, Li Dak Sum Yip Yio Chin Academic Building, City University of Hong Kong, Kowloon Tong, Kowloon, Hong Kong

School of Energy and Environment
City University of Hong Kong


Development of rapid and powerful mutagenesis and high throughput adaptive evolution tools is of importance for breeding of microbes aiming at application in environmental and industrial biotechnology. ARTP (atmospheric and room temperature plasma) mutagenesis system developed by our group can directly cause complex and diverse genome mutation including chain break and bases mutation via a unique mechanism. ARTP has the strongest DNA damage, and the highest mutation rate among the physical and chemical mutagenesis methods. Mechanistic study and practical application demonstrated that ARTP mutagenesis is a powerful tool for microbial evolution engineering to create diverse phenotypes. For developing an integrated platform capable of combing ARTP mutagenesis and high throughput adaptive evolution, we further developed a microdroplet-based microbial culture (MMC) system which can be operated automatically with high throughput culture on microchips, good repeatability, online detection of growth states, reprogrammable software, automatic addition of gradient chemical factors. The ARTP mutagenesis together with MMC system is an enabling platform for breeding of diverse microbes, especially aiming at application in open systems, such as environmental biotechnology and bioenergy, which require non-GM microorganisms.

About the Speaker

Prof. Xin-Hui XING received his B.S. from South China University of Technology in 1985, and Ph.D. from Tokyo Institute of Technology in 1992. He had been Assistant Professor at Tokyo Institute of Technology from 1992 to 1998, and Associate Professor at Yokohama National University from 1998 to2001. He was selected as a full professor by the 100-Telent Scholar Program of Tsinghua University in 2000 and joined Department of Chemical Engineering since then. He was appointed as the director of Institute of Biochemical Engineering in 2002, and deputy director of MOE Key Laboratory of Industrial Biocatalysis in 2011. He had been the vice department chairman from 2009 to 2018. His research field covers biobreeding technology and instrumentation, high throughput technology, enzyme engineering, environmental biotechnology and bioenergy. He serves as the editor of Journal of Bioscience and Bioengineering, associate editor of Biochemical Engineering Journal, and editorial board of several domestic and international journals.