Biophysical methods, especially at single-molecule level, is an essential tool in biomedical research and paves the way for numerous cutting-edge high-sensitivity, rapid diagnostic and detection techniques in healthcare. This course aims to provide students with an overview of fundamental concepts and methods in biophysics, including single-molecule dynamics and spectroscopy, protein folding, optical/magnetic tweezer, etc. After having a good understanding of the fundamental concepts and models, related applications in biomedicine and healthcare will be discussed, such as rapid diagnostic, ultrasensitive sensing for diseases, high throughput sequencing, point-of-care diagnostics, etc. Students will be able to learn critical knowledge and skills that can be used in their research, as well as in solving actual problems in healthcare field. A group project will be assigned at the end of the course for students to explore literatures for the most up-to-date progress of biophysical applications in healthcare.