Dr Minh Le (黎月明博士)

PhD (Singapore-MIT Alliance), Postdoc (Harvard Medical School)

Assistant Professor

Dr Minh Le

Contact Information

Office: 1B-110, 1/F, Block 1,
To Yuen Building
Lab: B2316, 2/F, Yeung Kin Man Academic Building
Phone: +852 3442-2485 (Office)
+852 3442-6203 (Lab)
Fax: +852 3442-0549
Email: mle.bms@cityu.edu.hk
Web: Personal Homepage
CityU Scholars

Research Interests

  • Cancer intercellular signaling
  • Cancer diagnostics and therapeutics

Dr Le graduated from the National University of Singapore in 2005 with a Bachelor degree in Life Sciences. She further received a Ph.D. degree in Computational and Systems Biology from the Singapore-Massachusetts Institute of Technology (MIT) Alliance under the guidance of Prof. Bing Lim at the Genome Institute of Singapore and Prof. Harvey Lodish at the Whitehead Institute for Biomedical Research. From 2010 to 2015, she worked as a postdoctoral fellow with Prof. Judy Lieberman at Boston Children’s Hospital and Harvard Medical School in the USA. She joined the Department of Biomedical Sciences at City University of Hong Kong (CityU) as a tenure-track Assistant Professor in August 2015.

Dr Le is well recognised for her contributions to the field of microRNAs (miRNAs) and cancer biology. She was the first to identify a miRNA that regulates p53, an important tumor suppressor gene. This miRNA, miR-125b, was subsequently found to be a potent oncogenic miRNA in leukemia and many solid tumors. Dr Le characterised the anti-apoptotic functions of miR-125b in zebrafish embryos and mammalian cells. She demonstrated that this tiny RNA is dispensable for normal development as it regulates the delicate balance between cell death and growth by repressing the p53 gene network. Furthermore, during her postdoctoral training, Dr Le illustrated a novel mechanism of cancer crosstalk in which miRNAs secreted by metastatic breast cancer cells are delivered to non-metastatic cells via extracellular vesicles (EVs) and promote colonization of tumor cells in the lung. With these original findings, Dr Le’s articles have been cited over 1200 times by researchers worldwide (link).

In addition to her interest in research, Dr Le also has a passion in education. She has organized the translation of “Molecular Cell Biology”, an American biology textbook by Lodish et al., into Vietnamese. As the project coordinator for the last 7 years, she has brought together the American authors, Vietnamese scholars, publishers, sponsors and hundreds of backers, to translate the most advanced knowledge in biology and promote science education in Vietnam. The first three volumes of the book are warmly welcomed by scientists and biology students in the country (link). Dr Le also organizes the Hong Kong RNA club for education in RNA research (link).

Dr Le was awarded several prestigious scholarships and fellowships during her studies such as the Lee Foundation study grant and the Singapore-MIT Alliance scholarship. She was one of the first three recipients of the L’Oréal Singapore for Women in Science National Fellowship broadcasted widely by the news in 2009 (link). During her training at Harvard Medical School, she was awarded the Jane Coffin Childs fellowship, a prestigious postdoctoral fellowship in the USA. She also won a number of competitive travel scholarships and poster awards at international conferences.

Research Interests

  1. Determine how EVs mediate the interaction of tumor cells with microenvironmental cells to promote drug resistance and cancer metastasis.
    Cancer is a critical disease with increasing incidence in Asia due to the increase in life expectancy and many other socioeconomic changes. In the last decades, early detection and treatment for certain types of cancers has significantly improved survival, but drug resistance and cancer metastasis remain the major cause of patient mortality. Metastasis is a multi-step process that involves the translocation of cancer cells to distant organs, and this requires tumor cells to constantly adapt to the changing microenvironments at each stage. During this process, tumor cells often release a large amount of bioactive molecules, including proteins, nucleic acids and lipids, into EVs which mediate their communication with other cells in the tumor niches and at distance sites. Although it is known that EVs secreted by tumor cells deliver bioactive molecules to various cell types, it is still unclear how these cargos alter recipient cell functions, thereby promoting metastasis. One of our research goals is to investigate the key molecular mechanisms of EV-mediated cancer communication in order to identify effective therapies against this disease.
     
  2. Develop EV-based therapies for cancer treatment.
    Based on the fact that EVs are natural vehicles of RNAs in intercellular communication, we have developed a strategy to harness EVs from red blood cells (RBCs) for delivery of RNA drugs to cancer cells. RBCs are readily available from blood banks and they release a large amount of EVs hence, it is easy to purify RBCEVs in large quantities without any expensive and labor-intensive cell culture. RBCEVs mediate robust delivery of therapeutic RNAs including antisense oligo nucleotides (ASOs), Cas9 mRNA and gRNAs to both leukemia cells and solid cancer cells for efficient miRNA inhibition and genome editing. Moreover, this delivery platform is safe as RBCEVs are devoid of DNA, growth factors, and toxic substances since human RBCs are enucleated primary cells. Our study is recently published in Nature Communications and patented by CityU. We are currently engineering RBCEVs further for specific delivery of gene therapies targeting different types of cancer.

Advantages of the RBCEV-based drug delivery platform (Le et al, Nature Research Bioengineering Community 2018)
Advantages of the RBCEV-based drug delivery platform (Le et al, Nature Research Bioengineering Community 2018)

Training Opportunities

We welcome BSc or MSc graduates with great interest in cancer research to contact us for research assistant positions and PhD scholarships:

  • Hong Kong PhD fellowship scheme (link)
  • Scholarship for the PhD programme in Biomedical Sciences (link)
  • Scholarship for the Interdisciplinary PhD programme in Veterinary Sciences in collaboration with Cornell University (link)

Candidates are required to have a good background in molecular cell biology (GPA > 8/10), good research experience, and fluent English (IELTS > 6 or TOEFL > 78).

Publications

Major Publications (# lead author, * corresponding author)

  • Ka Lung Chana#, Boya Peng#, Mubarak I. Umar, Chun Yin Chan, Aleksandr B. Sahakyan, Minh T.N. Le*, and Chun Kit Kwok*, Structural analysis reveals formation and role of RNA G-quadruplex structures in human mature microRNAs, Chemical Communications (2018) 54:10878-81.
  • Daniel Xin Zhang#, Theodoros Kiomourtzis#, Austin Lam, Minh Le TN*, “The biology and therapeutic applications of red blood cell extracellular vesicles” chapter in the book “Erythrocytes”, IntechOpen (accepted).
  • Muhammad Waqas#, Tin Chanh Pham, Yuk Yan Kwok, Luyen Tien Vu, Victor Ma, Boya Peng, Yuen San Chan, Likun Wei, Siew Mei Chin, Ajijur Azad, Alex Bai-Liang He, Anskar Y.H. Leung, Mengsu Yang, Ng Shyh-Chang, William C. Cho, Jiahai Shi, Minh TN Le*, Efficient RNA drug delivery using red blood cell extracellular vesicles; Nature Communications (2018) 9(1):2359. link
  • Camille A. Mathey-Andrews#, Minh Le TN*, Small but mighty: microRNAs as novel signaling molecules in cancer, RNA & Disease (2015) 2: e627. link
  • Minh Le TN#, Peter Hamar, Changying Guo, Emre Basar, Ricardo Henriques, Leonora Balaj, and Judy Lieberman*; miR-200 in extracellular vesicles promotes metastasis of breast cancer cells. Journal of Clinical Investigation (2014) 124: 5109–28 (highlighted by a commentary in the same issue and featured on Boson Children’s Hospital website). link
  • Minh Le TN#, Shyh-Chang Ng#, Swea Ling Khaw, Michael Chin, Cathleen Teh, Junliang Tay, Elizabeth O’Day, Vladimir Korzh, Henry Yang, Ashish Lal, Judy Lieberman, Harvey Lodish* and Bing Lim*; Conserved miR-125b regulation of p53 network dosage occurs through evolving microRNA-target gene pairs; PLoS Genetics (2011), e1002242. link
  • Minh Le TN#, Cathleen Teh, Shyh-Chang Ng, Huangming Xie, Beiyan Zhou, Vladimir Korzh, Harvey Lodish* and Bing Lim*; microRNA-125b is a novel negative regulator of p53; Genes & Development (2009) 23: 862-76 (featured on the Whitehead Institute’s website and in 16 other science news websites/magazines). link
  • Minh Le TN#, Huangming Xie, Beiyan Zhou, Poh HuiChia, Moonkyoung Um, Gerald Udolph, Henry Yang, Bing Lim* and Harvey Lodish*; microRNA-125b promotes neuronal differentiation in human cells by repressing multiple targets; Molecular and Cellular Biology (2009) 29: 5290-305 (featured as the most read article of the issue). link
  • Minh Le TN#, MA Reza, Sanjay Swarup and Manjunatha Kini*; Gene duplication of coagulation factor V and origin of venom prothrombin activator in Pseudonaja textilis snake; Thrombosis and Haemostasis (2005) 93: 420 – 429. link

Other publications

  • Ricardo Henriques#, Fabio Petrocca, Gabriel Altschuler, Marshall Thomas, Minh Le TN, Shen Mynn Tan, Winston Hide, Judy Lieberman*; miR-200 promotes the mesenchymal to epithelial transition by suppressing multiple members of the Zeb2 and Snail transcriptional repressor complexes. Oncogene, (2016) 35:158-72. link
  • Elizabeth O’Day#, Minh Le TN, Shunsuke Imai, Shen Mynn Tan, Rory Kirchner, Haribabu Arthanari, Oliver Hofmann, Gerhard Wagner, and Judy Lieberman*; An RNA-binding protein, Lin28, recognizes and remodels G-quartets in the microRNAs (miRNAs) and mRNAs it regulates. Journal of Biological Chemistry (2015) 290(29): 17909-22. link
  • Adi Gilboa Geffen#, Peter Hamar, Minh Le TN, Lee Adam Wheeler, Radiana Trifonova, Fabio Petrocca, Anders Wittrup, Judy Lieberman*; Gene knockdown by EpCAM aptamer-siRNA chimeras suppresses epithelial breast cancers and their tumor-initiating cells. Molecular Cancer Therapeutics, (2015) 14:2279-91. link
  • Jayoung Kim#, Samantha Morley, Minh Le TN, Denis Bedoret, Dale T. Umetsu, Dolores Di Vizio, and Michael R. Freeman*; Enhanced shedding of extracellular vesicles from amoeboid prostate cancer cells: Potential effects on the tumor microenvironment; Cancer Biology & Therapy (2014), 15: 409-418. link
  • Hung Nguyen#, Luong Nguyen, Minh Le TN, Le Trinh, Thao Nguyen, Diu Nguyen, Thuy Tran, Nhuong Nguyen, Hoang Le; Vietnamese translation of Molecular Cell Biology by Lodish et al., 7th edition; Tre publishing house; 2014 (reprinted in 2016). link
  • MA Reza#, Minh Le TN, Sanjay Swarup and Manjunatha Kini*; Molecular Evolution Caught In Action: Gene Duplication and Evolution of Molecular Isoforms of Prothrombin Activators In Pseudonaja textilis (Brown Snake); Journal of Thrombosis and Haemostasis (2006) 4: 1346-53. link

Funding

  • City University of Hong Kong Vice-president Research & Technology Grant, Oct 2015 – Sep 2019.
  • Hong Kong Health and Medical Research Fund Research Grant, Apr 2016 – Sep 2018.
  • City University of Hong Kong Applied Research Grant, May 2016 – Apr 2018.
  • Hong Kong Early Career Development Scheme Grant, Jan 2017 – Dec 2019.
  • Chinese National Natural Science Foundation Grant, Jan 2017 – Dec 2019.
  • City University of Hong Kong Teaching Grant, Jan 2017 – Jun 2018.
  • Chinese National Natural Science Foundation Grant, Jan 2018 – Dec 2020.

Teaching

  • BMS8107 – Cancer Biology & Precision Medicine (graduate course, semester B, Dr Le is the course leader)
  • BMS3007 – Good Laboratory Practice, Regulatory Compliance, and Ethical, Legal and Social Issues (undergraduate course, semester B, Dr Le is the course leader)
  • BMS8111 – Immunology & Infectious Diseases (graduate course, semester B)
  • BMS4001 – Medical Informatics and Laboratory Management (undergraduate course, semester B)
  • BMS8110 – Genomics and Bioinformatics (graduate course, semester A)